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crystal violet  (Beyotime)


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    Structured Review

    Beyotime crystal violet
    Crystal Violet, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 6755 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/staining+solution/Crystal+Violet+Staining+Solution/pm42893935-402-10-13
    Average 99 stars, based on 6755 article reviews
    crystal violet - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    Staining:

    Article Title: Liquiritin Enhances Chemosensitivity to Doxorubicin in Breast Cancer by Promoting Ubiquitination-Mediated Degradation of MCL1.
    Article Snippet: Breast cancer (BC) represents a life‐threatening malignant disease that profoundly endangers women's health, with chemoresistance restricting treatment efficacy.. Liquiritin (LIQ) has shown great potential in cancer therapy, yet its effect on the chemosensitivity of BC cells remains unclear.. BC cells were treated with different concentration gradients of Doxorubicin (DOX) and LIQ.

    Article Title: Epigenetically silenced KAT2B suppresses de novo lipogenesis through destroying HDAC5/LSD1 complex assembly in renal cell carcinoma
    Article Snippet: .. Dead or apoptotic cells in the organoids were stained using Beyo3DTM YO-PRO-1 (C1356S, Beyotime, China) staining solution, following the manufacturer's instructions. .. Six organoids were randomly selected from each group to analyze the ratio of dead cell area to the total organoid area using ImageJ software.

    Article Title: Degradation-Mediated Bioactive Calcium Release from Alginate Gel Fibers for Enhanced Bone Regeneration.
    Article Snippet: Biodegradable hydrogels are promising for bone regeneration but are limited by a significant mechanical mismatch with bone tissue in modulus, strength, and toughness.. To address the weakness, this work reported a mesh-like hydrogel woven from calcium-alginate gel fibers.. The fibers, manufactured via wet-spinning, possessed tensile strengths (36–285 MPa) and moduli (200 MPa–9.1 GPa) comparable to those of bone tissue.

    Article Title: RIPK3 overcomes 5-fluorouracil resistance in colorectal cancer cells via AKT/mTOR-mediated autophagy
    Article Snippet: .. For crystal violet staining, cells were incubated with staining solution (Beyotime, China) for 30 min, followed by gentle washing 3 times with PBS, air-drying, and final photography. .. Confluent cellular monolayers were established in six-well plates and transfected using Lipo 8000 transfection reagent (Beyotime Biotechnology, C0601).

    Article Title: Przewaquinone A regulates cell cycle and autophagy through the SrC/STAT3 signaling pathway to inhibit colorectal cancer progression.
    Article Snippet: Przewaquinone A (PrA), a natural active substance extracted from Salvia przewalskii Maxim, has been shown to have antitumor activity and can act as a STAT3 inhibitor to modulate the Src/STAT3 pathway.. However, its role in colorectal cancer (CRC), along with its related mechanisms, has not yet been clarified.. This study aims to explore whether PrA inhibits the malignant advancement of CRC via the Src/STAT3 pathway, and to provide new drug candidates and molecular targets for CRC clinical treatment.

    Article Title: Myeloperoxidase (MPO) mediates LPS-induced mitophagy in murine macrophages through AMPK/ULK1 pathway.
    Article Snippet: Neutrophils play a key role in innate immune system, taking up 50%70% of the whole circulating leukocytes, which are considered as the body's first line defense against pathogens.. Once reach the injured site, active neutrophils could release myeloperoxidase (MPO) through degranulation or neutrophil extracellular traps (NETs), making MPO a hallmark for neutrophil activation [1].. As a heme-containing peroxidase, MPO could kill pathogens through catalyzing H2O2 and halide ions (Cl , Br , I and SCN ) to produce oxidative hypohalides, among which hypochlorous acid (HOCl) is the main product in vivo [2,3].

    Article Title: Mechanistic study of locally transplanted bone marrow mesenchymal stem cells combined with erythropoietin in acute spinal cord injury.
    Article Snippet: .. The sections were stained with hematoxylin and eosin (H&E, Beyotime, China.) staining solution and observed under a microscope. .. The sections were stained with hematoxylin and eosin (H&E, Beyotime, China.)

    Article Title: PM2.5 exposure impairs BMSC function and dysregulates mitochondrial oxidative stress: multi-omics insights into potential osteoporotic mechanisms.
    Article Snippet: Background and objectives: Although PM2.5 exposure has been increasingly linked to osteoporosis, the underlying mechanisms remain incompletely understood.. This study aimed to investigate the toxic effects and molecular mechanisms of PM2.5 on bone marrow mesenchymal stem cells (BMSCs).. Methods: Rat BMSCs were exposed to PM2.5 Cytotoxicity, cytoskeletal integrity, migration, mitochondrial function, and osteogenic differentiation were assessed using a range of in vitro assays.

    CCK-8 Assay:

    Article Title: Degradation-Mediated Bioactive Calcium Release from Alginate Gel Fibers for Enhanced Bone Regeneration.
    Article Snippet: Biodegradable hydrogels are promising for bone regeneration but are limited by a significant mechanical mismatch with bone tissue in modulus, strength, and toughness.. To address the weakness, this work reported a mesh-like hydrogel woven from calcium-alginate gel fibers.. The fibers, manufactured via wet-spinning, possessed tensile strengths (36–285 MPa) and moduli (200 MPa–9.1 GPa) comparable to those of bone tissue.

    Incubation:

    Article Title: RIPK3 overcomes 5-fluorouracil resistance in colorectal cancer cells via AKT/mTOR-mediated autophagy
    Article Snippet: .. For crystal violet staining, cells were incubated with staining solution (Beyotime, China) for 30 min, followed by gentle washing 3 times with PBS, air-drying, and final photography. .. Confluent cellular monolayers were established in six-well plates and transfected using Lipo 8000 transfection reagent (Beyotime Biotechnology, C0601).

    Article Title: Myeloperoxidase (MPO) mediates LPS-induced mitophagy in murine macrophages through AMPK/ULK1 pathway.
    Article Snippet: Neutrophils play a key role in innate immune system, taking up 50%70% of the whole circulating leukocytes, which are considered as the body's first line defense against pathogens.. Once reach the injured site, active neutrophils could release myeloperoxidase (MPO) through degranulation or neutrophil extracellular traps (NETs), making MPO a hallmark for neutrophil activation [1].. As a heme-containing peroxidase, MPO could kill pathogens through catalyzing H2O2 and halide ions (Cl , Br , I and SCN ) to produce oxidative hypohalides, among which hypochlorous acid (HOCl) is the main product in vivo [2,3].

    Gentle:

    Article Title: RIPK3 overcomes 5-fluorouracil resistance in colorectal cancer cells via AKT/mTOR-mediated autophagy
    Article Snippet: .. For crystal violet staining, cells were incubated with staining solution (Beyotime, China) for 30 min, followed by gentle washing 3 times with PBS, air-drying, and final photography. .. Confluent cellular monolayers were established in six-well plates and transfected using Lipo 8000 transfection reagent (Beyotime Biotechnology, C0601).

    Infection:

    Article Title: Myeloperoxidase (MPO) mediates LPS-induced mitophagy in murine macrophages through AMPK/ULK1 pathway.
    Article Snippet: Neutrophils play a key role in innate immune system, taking up 50%70% of the whole circulating leukocytes, which are considered as the body's first line defense against pathogens.. Once reach the injured site, active neutrophils could release myeloperoxidase (MPO) through degranulation or neutrophil extracellular traps (NETs), making MPO a hallmark for neutrophil activation [1].. As a heme-containing peroxidase, MPO could kill pathogens through catalyzing H2O2 and halide ions (Cl , Br , I and SCN ) to produce oxidative hypohalides, among which hypochlorous acid (HOCl) is the main product in vivo [2,3].

    Microscopy:

    Article Title: Mechanistic study of locally transplanted bone marrow mesenchymal stem cells combined with erythropoietin in acute spinal cord injury.
    Article Snippet: .. The sections were stained with hematoxylin and eosin (H&E, Beyotime, China.) staining solution and observed under a microscope. .. The sections were stained with hematoxylin and eosin (H&E, Beyotime, China.)



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    Genetic and Pharmacological Inhibition of Nupr1 Attenuates Ang II Induced Mitochondrial Apoptosis in VSMCs. (A) Cell ability was evaluated by CCK8 assay ( n = 8 per group). (B) Lactate production of indicated groups ( n = 6 per group). (C) Representative images of TEM in VSMCs from the indicated groups. (D) Quantification of mitochondrial size in VSMCs from indicated groups ( n = 6 per group). (E) Quantification of circularity index in VSMC from indicated groups ( n = 6 per group). (F) Real‐time monitoring of oxygen consumption rate (OCR) in indicated groups detected by O2k. (G–I) Quantification of ATP‐coupled respiration, mitochondrial basal respiration, and maximal respiration from indicated groups ( n = 6 per group). (J) Representative images of <t>calcein</t> and Annexin V fluorescence results <t>showing</t> <t>mPTP</t> opening and apoptosis in indicated groups. Stronger green fluorescence represents a lower degree of mPTP opening, while diminished fluorescence indicates increased mPTP opening. Stronger red fluorescence represents a higher apoptosis rate. (K, L) Quantification of fluorescence intensity, including Annexin V and Calcein ( n = 6 per group). (M) Flow cytometry analysis of Annexin and PI staining of apoptosis from indicated groups. (N) Quantification of early apoptosis, late apoptosis, and total apoptosis by flow cytometry ( n = 3 per group). (O) Quantification of maximal respiration from indicated groups ( n = 6 per group). (P) Real‐time monitoring of OCR in indicated groups detected by O2k. (Q, R) Quantification of ATP‐coupled respiration and mitochondrial basal respiration from indicated groups ( n = 6 per group). (S) Flow cytometry analysis of Annexin and PI staining of apoptosis from indicated groups. (T) Quantification of early apoptosis, late apoptosis, and total apoptosis by flow cytometry ( n = 3 per group).
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    Image Search Results


    Genetic and Pharmacological Inhibition of Nupr1 Attenuates Ang II Induced Mitochondrial Apoptosis in VSMCs. (A) Cell ability was evaluated by CCK8 assay ( n = 8 per group). (B) Lactate production of indicated groups ( n = 6 per group). (C) Representative images of TEM in VSMCs from the indicated groups. (D) Quantification of mitochondrial size in VSMCs from indicated groups ( n = 6 per group). (E) Quantification of circularity index in VSMC from indicated groups ( n = 6 per group). (F) Real‐time monitoring of oxygen consumption rate (OCR) in indicated groups detected by O2k. (G–I) Quantification of ATP‐coupled respiration, mitochondrial basal respiration, and maximal respiration from indicated groups ( n = 6 per group). (J) Representative images of calcein and Annexin V fluorescence results showing mPTP opening and apoptosis in indicated groups. Stronger green fluorescence represents a lower degree of mPTP opening, while diminished fluorescence indicates increased mPTP opening. Stronger red fluorescence represents a higher apoptosis rate. (K, L) Quantification of fluorescence intensity, including Annexin V and Calcein ( n = 6 per group). (M) Flow cytometry analysis of Annexin and PI staining of apoptosis from indicated groups. (N) Quantification of early apoptosis, late apoptosis, and total apoptosis by flow cytometry ( n = 3 per group). (O) Quantification of maximal respiration from indicated groups ( n = 6 per group). (P) Real‐time monitoring of OCR in indicated groups detected by O2k. (Q, R) Quantification of ATP‐coupled respiration and mitochondrial basal respiration from indicated groups ( n = 6 per group). (S) Flow cytometry analysis of Annexin and PI staining of apoptosis from indicated groups. (T) Quantification of early apoptosis, late apoptosis, and total apoptosis by flow cytometry ( n = 3 per group).

    Journal: Advanced Science

    Article Title: Targeting Nupr1‐Mediated p53 Mitochondrial Sequestration Alleviates Aortic Dissection by Restoring the Gadd45b‐Dependent Survival Program

    doi: 10.1002/advs.78143

    Figure Lengend Snippet: Genetic and Pharmacological Inhibition of Nupr1 Attenuates Ang II Induced Mitochondrial Apoptosis in VSMCs. (A) Cell ability was evaluated by CCK8 assay ( n = 8 per group). (B) Lactate production of indicated groups ( n = 6 per group). (C) Representative images of TEM in VSMCs from the indicated groups. (D) Quantification of mitochondrial size in VSMCs from indicated groups ( n = 6 per group). (E) Quantification of circularity index in VSMC from indicated groups ( n = 6 per group). (F) Real‐time monitoring of oxygen consumption rate (OCR) in indicated groups detected by O2k. (G–I) Quantification of ATP‐coupled respiration, mitochondrial basal respiration, and maximal respiration from indicated groups ( n = 6 per group). (J) Representative images of calcein and Annexin V fluorescence results showing mPTP opening and apoptosis in indicated groups. Stronger green fluorescence represents a lower degree of mPTP opening, while diminished fluorescence indicates increased mPTP opening. Stronger red fluorescence represents a higher apoptosis rate. (K, L) Quantification of fluorescence intensity, including Annexin V and Calcein ( n = 6 per group). (M) Flow cytometry analysis of Annexin and PI staining of apoptosis from indicated groups. (N) Quantification of early apoptosis, late apoptosis, and total apoptosis by flow cytometry ( n = 3 per group). (O) Quantification of maximal respiration from indicated groups ( n = 6 per group). (P) Real‐time monitoring of OCR in indicated groups detected by O2k. (Q, R) Quantification of ATP‐coupled respiration and mitochondrial basal respiration from indicated groups ( n = 6 per group). (S) Flow cytometry analysis of Annexin and PI staining of apoptosis from indicated groups. (T) Quantification of early apoptosis, late apoptosis, and total apoptosis by flow cytometry ( n = 3 per group).

    Article Snippet: According to mPTP test kit instructions (C2009S, Beyotime), 200 μL of Calcein AM staining solution and fluorescence quenching working solution were added to each well after the respective cell treatments.

    Techniques: Inhibition, CCK-8 Assay, Real-time Monitoring, Fluorescence, Flow Cytometry, Analysis, Staining

    Gadd45b is Indispensable for the Protective Effects of Nupr1 Deficiency Against Mitochondrial Apoptosis. (A) Enrichment of KEGG pathways. (B) GSEA analysis in VSMCs treated with Ang II stimulation and Nupr1 deficiency. (C) Chordal diagram of GO pathways related with cell fate and the contributing DEPs. (D) Stem plot of DEPs in the apoptosis pathway from the indicated groups. (E) RT‐qPCR analysis of mRNA levels of candidate genes in VSMCs. Data were presented as relative fold change to controls ( n = 6 per group). (F) Expressions of Gadd45b in VSMCs measured by Western blot and its quantification from indicated groups measured by Western blot ( n = 5 per group). (G, H) Representative images of ROS staining in indicated groups. Semi‐quantitative analysis of mean fluorescence intensity in indicated groups ( n = 6 per group). (I) Representative images of calcein and Annexin V fluorescence results showing mPTP opening in indicated groups. (J) Quantification of fluorescence intensity, including Annexin V and mPTP ( n = 3 per group). (K) Co‐immunoprecipitation and Western blot assays of Myc‐Nupr1 and Gadd45b. (L) Co‐immunoprecipitation assays of Myc‐Gadd45b and Nupr1.

    Journal: Advanced Science

    Article Title: Targeting Nupr1‐Mediated p53 Mitochondrial Sequestration Alleviates Aortic Dissection by Restoring the Gadd45b‐Dependent Survival Program

    doi: 10.1002/advs.78143

    Figure Lengend Snippet: Gadd45b is Indispensable for the Protective Effects of Nupr1 Deficiency Against Mitochondrial Apoptosis. (A) Enrichment of KEGG pathways. (B) GSEA analysis in VSMCs treated with Ang II stimulation and Nupr1 deficiency. (C) Chordal diagram of GO pathways related with cell fate and the contributing DEPs. (D) Stem plot of DEPs in the apoptosis pathway from the indicated groups. (E) RT‐qPCR analysis of mRNA levels of candidate genes in VSMCs. Data were presented as relative fold change to controls ( n = 6 per group). (F) Expressions of Gadd45b in VSMCs measured by Western blot and its quantification from indicated groups measured by Western blot ( n = 5 per group). (G, H) Representative images of ROS staining in indicated groups. Semi‐quantitative analysis of mean fluorescence intensity in indicated groups ( n = 6 per group). (I) Representative images of calcein and Annexin V fluorescence results showing mPTP opening in indicated groups. (J) Quantification of fluorescence intensity, including Annexin V and mPTP ( n = 3 per group). (K) Co‐immunoprecipitation and Western blot assays of Myc‐Nupr1 and Gadd45b. (L) Co‐immunoprecipitation assays of Myc‐Gadd45b and Nupr1.

    Article Snippet: According to mPTP test kit instructions (C2009S, Beyotime), 200 μL of Calcein AM staining solution and fluorescence quenching working solution were added to each well after the respective cell treatments.

    Techniques: Analysis, Quantitative RT-PCR, Western Blot, Staining, Fluorescence, Co-Immunoprecipitation Assay, Significance Assay